The purpose of this pilot project is to investigate the relationship between the implementation of classification and labelling of chemical substances and their use. The system that has been examined is the EU legislation for classification and labelling of chemical substances and mixtures. The focus lies on substances with harmonised classification at European Union (EU) Community level as dangerous for the environment which by themselves or in a mixture above a certain concentration limit lead to classification and labelling with the symbol “Fish and tree” and the indication of danger Dangerous for the environment (N). Other effects were also included to some extent. EU Environmental hazard classification has been conducted since the mid 1990s and from the early first decade of the 21st century for chemical substances and mixtures. Now, almost 20 years after the first criteria were implemented it is interesting to look back and see whether this classification has had an impact on the use of these substances.